Part:BBa_K5127026
Constitutive promoter with IAA degradation enzyme
This composite part combines the constitutive promotor J23119 and transcriptional unit iadCDE.
Team: BNDS-China 2024
Design
To optimize the IAA degradation efficiency, we constructed a second plasmid that replaces the inducible T7 promoter with the constitutive promoter J23119 (Figure 1). This modification ensures continuous expression of the IAA degradation pathway, potentially enhancing its overall effectiveness.
Figure 1. Plasmid design of J23119-iadCDE. Created by biorender.com.
Figure 2. The agarose gel electrophoresis results of the PCR products of J23119-iadCDE construction. A, materials to construct J23119-iadCDE. B, Golden gate assembly result of J23119-iadCDE construction. The band at 7947bp in (B) indicated the success in plasmid construction.
We require additional time to further characterize and evaluate the degradation function to ensure optimal performance and reliability in our system.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2402
Illegal PstI site found at 2219
Illegal PstI site found at 2797 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2402
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 724
Illegal PstI site found at 2219
Illegal PstI site found at 2797 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2402
Illegal BglII site found at 2411
Illegal BglII site found at 2463 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2402
Illegal PstI site found at 2219
Illegal PstI site found at 2797 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2402
Illegal PstI site found at 2219
Illegal PstI site found at 2797
Illegal NgoMIV site found at 670
Illegal NgoMIV site found at 1661
Illegal AgeI site found at 434
Illegal AgeI site found at 1463
Illegal AgeI site found at 1904
Illegal AgeI site found at 2327
Illegal AgeI site found at 2761 - 1000COMPATIBLE WITH RFC[1000]
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